A natural ribozyme with 3′,5′ RNA ligase activity
نویسندگان
چکیده
منابع مشابه
Reconciling Ligase Ribozyme Activity with Fatty Acid Vesicle Stability
The "RNA world" and the "Lipid world" theories for the origin of cellular life are often considered incompatible due to the differences in the environmental conditions at which they can emerge. One obstacle resides in the conflicting requirements for divalent metal ions, in particular Mg2+, with respect to optimal ribozyme activity, fatty acid vesicle stability and protection against RNA strand...
متن کاملPrebiotic Factors Influencing the Activity of a Ligase Ribozyme
An RNA-lipid origin of life scenario provides a plausible route for compartmentalized replication of an informational polymer and subsequent division of the container. However, a full narrative to form such RNA protocells implies that catalytic RNA molecules, called ribozymes, can operate in the presence of self-assembled vesicles composed of prebiotically relevant constituents, such as fatty a...
متن کاملA self-replicating ligase ribozyme.
A self-replicating molecule directs the covalent assembly of component molecules to form a product that is of identical composition to the parent. When the newly formed product also is able to direct the assembly of product molecules, the self-replicating system can be termed autocatalytic. A self-replicating system was developed based on a ribozyme that catalyzes the assembly of additional cop...
متن کاملKinetic framework for ligation by an efficient RNA ligase ribozyme.
The class I RNA ligase ribozyme, isolated previously from random sequences, performs an efficient RNA ligation reaction. It ligates two substrate RNAs, promoting the attack of the 3'-hydroxyl of one substrate upon the 5'-triphosphate of the other substrate with release of pyrophosphate. This ligation reaction has similarities to the reaction catalyzed by RNA polymerases. Using data from steady-...
متن کاملDeoxyribozymes with 2'-5' RNA ligase activity.
In vitro selection was used to identify deoxyribozymes that ligate two RNA substrates. In the ligation reaction, a 2'-5' RNA phosphodiester linkage is created from a 2',3'-cyclic phosphate and a 5'-hydroxyl group. The new Mg(2+)-dependent deoxyribozymes provide 50-60% yield of ligated RNA in overnight incubations at pH 7.5 and 37 degrees C, and they afford 40-50% yield in 1 h at pH 9.0 and 37 d...
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ژورنال
عنوان ژورنال: Nature Chemical Biology
سال: 2009
ISSN: 1552-4450,1552-4469
DOI: 10.1038/nchembio.136